Figure 4.

Recovery of Yme1 siRNA–transfected cells from CCCP-induced mitochondrial fragmentation. (A–F) Cells were transfected with scrambled (A–C) or Yme1 siRNA oligonucleotides (D–F). Mitochondrial morphology was observed by cotransfection with mitochondrial targeted DsRed. Cells was observed without further treatment at 2 d after transfection (A and D), after incubation with CCCP for 60 min (B and E), or incubation with CCCP for 60 min followed by washout and 3.5-h recovery (C and F). (G) Percentages of cells with different mitochondrial morphologies and the treatments described in A–F. Morphologies were classified as connected (highly interconnected), normal (tubular morphology), intermediate (partially fragmented), and fragmented (fully fragmented). Samples sizes for the scrambled controls were as follows: n = 506 cells counted before the addition of CCCP, n = 246 counted after adding CCCP, and n = 100 cells counted at 3.5 h after washout. Sample sizes for Yme1 siRNA were n = 384, n = 332, and n = 169, respectively. Bar, 10 μm.