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APQ12 encodes a transmembrane protein that, when absent, results in cold sensitivity. (A) Differential centrifugation to determine membrane association. In addition to antibodies against GFP, the blot was also probed using antibodies directed against a control integral membrane protein (Bos1) as well as a peripherally associated membrane protein, Sec23. (B) Growth assays comparing WT and apq12Δ cells at various temperatures after 2 and 6 d. T, total; S, soluble; P, pellet.
