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apq12Δ cell growth rates correlate with Nup localization and function. (A) Images of WT and apq12Δ cells processed for either FISH assays or indirect IF using α-Nup159/Rat7 antibodies after overnight growth at 37°C. (B) WT or apq12Δ cells expressing either NUP60- or NUP170-GFP from the proper chromosomal locus were grown overnight at 30°C, shifted to 16°C, and viewed after either 1 or 2 d.
