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Apq12 is required for optimal NPC biogenesis. (A) WT or apq12Δ cells expressing Nup82-GFP were grown overnight at 37°C and either maintained at 37°C or shifted to 23°C with or without the translational inhibitor cycloheximide. Cells were then imaged after 8 h. (B) WT and apq12Δ cells lacking NUP120 were grown overnight at 23°C and processed for indirect IF using both α-Nup159/Rat7 and α-RL1 antibodies.
