Figure 3.

AnkG is required for AIS formation. (A) 10-DIV hippocampal neurons transfected with Nav channel shRNA (Nav1.x shRNA), ankG shRNA, or βIV spectrin shRNA, and immunolabeled for Nav channels (pan-Nav), ankG, βIV spectrin, NF (pan-NF), or NrCAM (red), and MAP2 (blue). GFP fluorescence indicates transfected neurons. Asterisks indicate untransfected neurons. (B) The ratio of GFP⁺ neurons labeled at the AIS (for the protein indicated) to the total number of GFP⁺ neurons. In a minimum of three independent experiments, 25–30 GFP⁺ positive neurons were counted per shRNA. Error bars indicate ± SD. Bar, 10 μm.