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. 2006 Oct 23;175(2):237–247. doi: 10.1083/jcb.200605138

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Dynamic redistribution of mitochondrial proteins involved in protein translocation and synthesis. Cells overexpressing mtGFP were or were not treated with puromycin for 30 min before fixation and immuno-EM. (A) Quantification of Tim23 distribution before or after 30 min of puromycin treatment. The experiment was repeated three times using identical fixed cell material. Error bars represent the SD. (B) Distribution of Mrpl36 before the addition of puromycin. (C) Distribution of Mrpl36 30 min after the addition of puromycin. (D) Location of Mrpl36 in the OM/IBM, CM, or matrix before and 30 min after the addition of puromycin. Numbers at x and y axes represent distances in nanometers.