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. 2006 Oct 23;175(2):249–259. doi: 10.1083/jcb.200605162

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — The transmembrane helix of atTic40 is required for membrane targeting. (A) [³⁵S]-labeled pre-atTic40 (atTic40), mature atTic40 fused to the transit peptide of SSU (psTP-Tic40), or a deletion mutant of pre-atTic40 lacking its transmembrane segment (ΔTM) were imported into chloroplasts for 30 min. After import, the chloroplasts were treated with thermolysin, reisolated, and total (T), membrane (M), or soluble (S) fractions were analyzed by SDS-PAGE and phosphorimaging. IVT, 20% of the in vitro–translated protein used in each reaction. (B) Quantitative analysis of distribution of each protein between the membrane and soluble fractions of chloroplasts after import. N.D. indicates that the sample was below accurate detection levels.