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. 2006 Oct 23;175(2):261–270. doi: 10.1083/jcb.200605196

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — shRNAs targeting SEL1L impair US11-mediated dislocation of class I MHC HC. (a) US11 cells were transduced with virus encoding shRNAs against GFP (control cells) or against SEL1L (knockdown cells) and analyzed for levels of SEL1L by immunoblotting with anti-SEL1L antibodies and anti–protein disulfide isomerase (PDI) antibodies as a loading control. (b) Control and knockdown cells were pulse-labeled for 10 min and chased for the indicated time points. Immunoprecipitation from SDS lysates for class I MHC HC (1), US11 (2), and HRD1 (3) were performed using respective antibodies. (c) Quantitation of the amount of glycosylated to deglycosylated HC from shRNA GFP (control) and shRNA SEL1L (knockdown) cells treated with ZL₃VS.