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. 2006 Oct 23;175(2):261–270. doi: 10.1083/jcb.200605196

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — SEL1L interacts with RI₃₃₂ 293T cells transfected with RI₃₃₂ that were pulse-labeled to steady-state for 5 h in the presence of 10 μM of the proteasome inhibitor ZL₃VS. Immunoprecipitations from digitonin lysates were performed with anti-SEL1L antibodies. The anti-SEL1L immunoprecipitate was either analyzed directly (b, lane 1) or reimmunoprecipitated sequentially using anti-RI I antibodies (b, lane 2) or anti-SEL1L antibodies (b, lane 3). (a) Input levels of endogenous RI and overexpressed RI₃₃₂. Immunoprecipitates were resolved on a 12% SDS polyacrylamide gel. Asterisk represents a presumably processed version of RI₃₃₂ in the ER.