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. 2006 Oct 23;175(2):261–270. doi: 10.1083/jcb.200605196

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — TCRα degradation is moderately slowed by shRNAs targeting SEL1L. The same control and knockdown cells used in Fig. 5 were transiently transfected with TCRα. 36 h after transfection these cells were pulse-labeled for 20 min and chased for the indicated time points. Immunoprecipitation from SDS lysates were performed using anti–TCRα-antibodies. (c) The histogram shows mean quantitation of two independent experiments.