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. 2006 Oct 23;175(2):271–281. doi: 10.1083/jcb.200606050

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — Loss of mature melanosomes but not stage II organelles in Rab38/Rab32-deficient cells. (A) Cht melanocytes subjected to two rounds of transfection with either control or Rab32-specific siRNA oligos were fixed, permeabilized, and labeled for Pmel17 with antibody HMB45. Note the reduction in pigmented structures in the corresponding brightfield images of Rab32 siRNA–treated cells. (B) Cht cells transfected with control siRNA were processed for conventional EM. Melanosomes at different stages of maturation are indicated. (C) Cht cells transfected with Rab32-specific siRNA were analyzed as in B. The inset shows immunogold labeling for Pmel17 on stage II melanosomes in cht cells treated with Rab32-specific siRNA and processed for immuno-EM. N, nucleus; GA, Golgi. Bars: (B and C) 500 nm; (inset) 200 nm.