Table I.
NLSs cloned as GFP fusion proteins for this study
| Protein | Function | NLS Sequence | Import Kapa | Reference |
|---|---|---|---|---|
| Rpl25p | Ribosome | MAPSAKATAAKKAVVKGTNGKKALKVRTSATFRLPKTLKLARAPK (aa 1–45) | Kap123p | Schaap et al., 1991 |
| Rps1bp | Ribosome | MAVGKNKRLSRGKKGLKKKVVDPFTRKEWFDIKAPSTFENRNVGKTLVNK(aa 1–50) | Kap123p | This study |
| Mak16p | Ribosome assembly | NKNSAKRRKKGTSAKTKRPKV(aa 267–287) | Unknown | This study |
| Nug1p | Ribosome assembly | MRVRKRQSRRTSTKLKEGIKKKASAHRKKEKKMAKKDVTWRSRSKKDPGIPSNFPYKAKIL (aa 1–61) | Kap123p/ Unknown | This study |
| Yil096cp | Ribosome assembly | MARKLKGKIGSKGLKGALLRHKAKVKLVRNIESKQKHELRKKNSSANNKTVKRNQEFQK- LNQGKVMP (aa 1–67) | Kap123p/ Unknown | This study |
| Yra1p | mRNA export | GSNKAGSNRARVGGTRGNGPRRVGKQVGSQRRSLPNRRGPIRKNTRAPPNAVARVAK (aa 14–70) | Kap121p/ Kap123p | Zenklusen et al., 2001; this study |
| Nab2p | mRNA binding | DNSQRFTQRGGGAVGKNRRGGRGGNRGGRNNNSTRFNPLAKALGMAGESN (aa 201–250) | Kap104p | Lee and Aitchison, 1999 |
| Pho4p | Phosphate regulation | ANKVTKNKSNSSPYLNKRRGKPGPDSATSLFELPDSVIPTPKPKPKPKQYPKVILP (aa 141–196) | Kap121p | Kaffman et al., 1998; this study |
a
According to references; also see Fig. 2, Fig. 3, and Fig. S3 (available at http://www.jcb.org/cgi/content/full/jcb.200608141/DC1).