Table II.

Summary of measured import statistics

Cargo	Rpl25NLS-GFP-PrA	Rpl25NLS-GFP-PrA	Pho4NLS-GFP-PrA	Nab2NLS-GFP-PrA
Cell type	Wild type	Δkap123	Wild type	Wild type
Primary Kap	Kap123	Kap121	Kap121	Kap104
Mean nuclear volume (fL)a	3.8 ± 1.4	3.2 ± 1.5	2.6 ± 1	2.9 ± 1.2
Mean cytoplasmic volume (fL)a	36 ± 17	35 ± 19	27 ± 12	30 ± 14
Mean number of NPCsa	142 ± 37	127 ± 42	110 ± 28	121 ± 33
Initial NLS-GFP concentration range (μM)b	7.2–109	6–104	3.6–68	4–58
Mean Kap concentration (μM)	5.3	1.0	1.0	0.6
Effective import rate (cargo molecules NPC−1 s−1 μM−1 cargo)c	1.2 ± 0.1	0.07 ± 0.01	0.10 ± 0.01	0.18 ± 0.02
K d Kap–NLS (nM)	94	82	93	17
Kap half saturation (μM)	7	7	n.d.	n.d.
Estimated bulk cargo concentration (μM; 20 nM K d)	5	5	5	0
Estimated nonspecific competitors concentration (μM; 500 nM K d ) e	1,000	1,000	1,000	1,000
k' (cargo molecules μM−1 NPC−1 s−1)c	44 ± 8	24 ± 8d	39 ± 5	22 ± 4d
p (cargo molecules μM−1 NPC−1 s−1)c	0.05 ± 0.01	0.06 ± 0.01	0.06 ± 0.01	0.064 ± 0.007
k (cargo molecules NPC−1 s−1)	300	300	n.d.	n.d.
KS (μM)	1	1	n.d.	n.d.

^a± SD.

^b5th to 95th interquartile range.

^c± 95% confidence interval for mean.

^dHighly significant difference from Rpl25NLS-GFP-PrA in wild-type cells.

^eSee discussion.