Table I.

Quantitative analysis of colocalization of LAPTM5 with organelle markers

Cell type	Organelle	Pearson's coefficient
HEK293T
WT	Lysosomes	0.819, r(20), P < 0.01
	Golgi	0.296, r(20), P > 0.10
3YA	Lysosomes	0.241, r(20), P > 0.10
	Golgi	0.809, r(20), P < 0.01
	Recycling e	0.118, r(15), P > 0.10
	Sorting e	0.103, r(15), P > 0.10
	Early e	0.104, r(15), P > 0.10
10KR	Lysosomes	0.733, r(20), P < 0.01
	Golgi	0.419, r(20), P > 0.05
	Recycling e	0.151, r(15), P > 0.10
	Sorting e	0.165, r(15), P > 0.10
	Early e	0.157, r(15), P > 0.10
UIMm	Lysosomes	0.410, r(20), P > 0.05
	Golgi	0.680, r(20), P < 0.01
	Recycling e	0.165, r(15), P > 0.10
	Sorting e	0.125, r(15), P > 0.10
	Early e	0.133, r(15), P > 0.10
WT + Nedd4 RNAi	Lysosomes	0.359, r(20), P > 0.10
	Golgi	0.783, r(20), P < 0.01
WT + GGA3 RNAi	Lysosomes	0.318, r(20), P > 0.10
	Golgi	0.748, r(20), P < 0.01
DC2.4
WT	Lysosomes	0.712, r(20), P < 0.01
	Golgi	0.218, r(20), P > 0.10
3YA	Lysosomes	0.398, r(25), P < 0.05
	Golgi	0.453, r(25), P < 0.05
	Recycling E	0.165, r(15), P > 0.10
	Sorting E	0.167, r(15), P > 0.10
	Early E	0.162, r(15), P > 0.10

Quantification on individual randomly selected dsRed-LAPTM5–transfected cells was performed using LSM510 and Volocity 3.7.0. The extent of colocalization is indicated as Pearson's coefficient, corresponding to the degrees of freedom (r) and level of significance (P). The following markers of organelles were used in the analysis: LAMP1 (lysosomes), giantin (Golgi), Rab11 (recycling endosomes), Hrs (sorting endosomes), and EEA1 (early endosomes).