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. 2006 Nov 20;175(4):647–659. doi: 10.1083/jcb.200605001

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — ΔNCEA restores differentiation to NCB-expressing cells. (A) Photomicrographs of L6 transfectants tested for myogenic differentiation after a 7-d incubation in DM. Parental and ΔNCEA cells fused readily, whereas NCB and CEA completely blocked differentiation; coexpression of ΔNCEA with NCB restored differentiation. (B) Up-regulation of the differentiation marker myosin, as determined by Western blot with mAb 47A on 10 μg of total lysate, was seen only in parental, ΔNCEA, and two separate populations of ΔNCEA + NCB. No myosin expression was seen in exponentially growing (exp) cultures of NCB or ΔNCEA + NCB transfectants. (C) NCB expression in differentiated cultures. Western blots of 10 μg of total cellular lysate demonstrated that NCB expression remained higher in coexpressing cells than in NCB-alone transfectant cells after 7 d in DM.