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. 2006 Nov 20;175(4):647–659. doi: 10.1083/jcb.200605001

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Increased ECM binding by NCB transfectants is lost in the presence of ΔNCEA. (A) L6 (CEA) and L6 (NCB) transfectants showed increased binding, compared with parental L6 cells, to immobilized Fn and Vn, whereas the presence of ΔNCEA in NCB transfectants abrogated this increase. No difference in collagen I binding was seen between parental and transfectant lines. Mean values are ± the SD from three independent experiments are shown (*, P < 0.004). (B) LR transfectants showed a similar pattern of binding to Fn, with an increase seen for NCB, but not for ΔNCEA + NCB transfectants (*, P < 0.003). (C) No significant alteration in integrin β1 (top) or α5 (bottom) expression was seen by Western blots on 5 μg of lysate from each L6 cell line.