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. 2007 Sep 10;178(6):913–924. doi: 10.1083/jcb.200702058

Figure 4.

Figure 4.

Strip-FRAP analysis of GFP, GFP-UTF1, H2B-GFP, and Oct4-GFP. (A) FRAP analysis of EC cells expressing GFP (green line) or GFP-UTF1 (blue line). The graph shows the relative fluorescent recovery directly after bleaching. The prebleach level is normalized to 1. GFP displays a quick fluorescent recovery in the bleached region, whereas GFP-UTF1 shows only little recovery directly after photobleaching. (B) FRAP analysis of ES cells expressing GFP (green line) or GFP-UTF1 (blue line). GFP shows a quick recovery after bleaching, whereas GFP-UTF1 displays only a marginal recovery. (C) FRAP experiment of EC cells expressing either GFP-UTF1 (blue line) or histone H2B-GFP (red line). GFP-UTF1 and H2B-GFP both show only a small recovery directly after photobleaching. (D) FRAP experiment of EC cells expressing GFP (green line), GFP-UTF1 (blue line), or Oct4-GFP (red line). Oct4-GFP shows a quick recovery after fluorescence, although slower than GFP.