Table I.
Modulation of axonal mRNA levels in response to growth-promoting and growth-inhibiting stimuli
| mRNA | NGF | BDNF | NT3 | MAG | Sema3A |
|---|---|---|---|---|---|
| αB crystallin | 1.22 ± 0.10 | 1.17 ± 0.08 | 1.20 ± 0.16 | 4.59 ± 0.24 | 5.03 ± 0.13 |
| Aldolase C | 1.04 ± 0.07 | −1.09 ± 0.04 | −1.75 ± 0.14 | −1.30 ± 0.25 | −1.52 ± 0.21 |
| Amphoterin | 1.03 ± 0.19 | 1.04 ± 0.14 | 1.05 ± 0.18 | −1.03 ± 0.16 | −1.08 ± 0.15 |
| ATP synthase | 1.31 ± 0.07 | 1.06 ± 0.09 | −1.92 ± 0.14 | 1.06 ± 0.09 | 1.21 ± 0.22 |
| β-actin | 4.14 ± 0.48 | 4.41 ± 0.38 | 2.17 ± 0.13 | −1.30 ± 0.16 | −1.38 ± 0.19 |
| CACNA1 | −1.45 ± 0.11 | −1.56 ± 0.12 | −1.19 ± 0.14 | 1.12 ± 0.09 | 1.28 ± 0.21 |
| Calmodulin (RCM3) | 1.13 ± 0.18 | 1.05 ± 0.25 | −1.00 ± 0.16 | 1.10 ± 0.18 | 1.08 ± 0.20 |
| Calreticulin | 1.06 ± 0.10 | 1.21 ± 0.40 | 2.22 ± 0.06 | 2.73 ± 0.15 | 4.20 ± 0.09 |
| Cathepsin B | −1.04 ± 0.12 | −1.06 ± 0.15 | 1.01 ± 0.11 | 1.04 ± 0.13 | 1.06 ± 0.17 |
| Cofilin | 1.13 ± 0.05 | 1.04 ± 0.09 | 1.19 ± 0.06 | −1.06 ± 0.09 | −1.03 ± 0.08 |
| CsA | 1.15 ± 0.06 | −1.07 ± 0.19 | −1.54 ± 0.06 | 1.13 ± 0.12 | −1.31 ± 0.07 |
| Cyclophilin A | 1.07 ± 0.16 | 1.04 ± 0.20 | 1.06 ± 0.17 | −1.03 ± 0.16 | −1.18 ± 0.15 |
| Cystatin C | 1.13 ± 0.12 | 1.02 ± 0.14 | −1.04 ± 0.15 | 1.01 ± 0.20 | 1.01 ± 0.16 |
| Ddah2 | −1.03 ± 0.13 | −1.07 ± 0.15 | −1.52 ± 0.16 | 1.43 ± 0.11 | 1.13 ± 0.02 |
| Enolase | 1.32 ± 0.08 | 1.35 ± 0.14 | −1.05 ± 0.09 | 1.19 ± 0.12 | −1.58 ± 0.19 |
| ERp29 | −1.01 ± 0.08 | 1.03 ± 0.07 | 1.17 ± 0.09 | 1.18 ± 0.09 | −1.08 ± 0.09 |
| GAP43 | 2.25 ± 0.06 | 1.13 ± 0.13 | −1.01 ± 0.05 | −1.09 ± 0.08 | −1.13 ± 0.08 |
| GAPDH | 1.07 ± 0.09 | 1.03 ± 0.04 | −1.03 ± 0.13 | −1.01 ± 0.19 | −1.05 ± 0.12 |
| grp75 | −1.15 ± 0.07 | −1.05 ± 0.07 | 1.24 ± 0.20 | 1.09 ± 0.16 | 1.14 ± 0.08 |
| grp78/BiP | −1.03 ± 0.07 | 1.05 ± 0.10 | 1.23 ± 0.08 | 1.67 ± 0.15 | 1.47 ± 0.22 |
| γ-Synuclein | −1.03 ± 0.07 | 1.02 ± 0.13 | −1.56 ± 0.05 | 1.01 ± 0.15 | −1.01 ± 0.06 |
| γ-Tropomyosin 3 | 1.03 ± 0.07 | 1.03 ± 0.07 | −1.47 ± 0.06 | 1.13 ± 0.10 | −1.69 ± 0.05 |
| HCN4 | −1.40 ± 0.17 | −1.35 ± 0.09 | 1.17 ± 0.21 | 1.21 ± 0.16 | 2.07 ± 0.21 |
| hnRNPH′ | 1.02 ± 0.06 | 1.03 ± 0.10 | −1.03 ± 0.08 | 1.23 ± 0.15 | −1.13 ± 0.13 |
| HSP27 | 1.08 ± 0.21 | −1.03 ± 0.16 | −1.56 ± 0.08 | −1.44 ± 0.14 | 1.05 ± 0.09 |
| HSP60 | −1.13 ± 0.07 | −1.13 ± 0.09 | 2.36 ± 0.23 | 1.46 ± 0.13 | 2.45 ± 0.21 |
| HSP70 | 1.01 ± 0.06 | −1.09 ± 0.05 | −2.27 ± 0.13 | −1.10 ± 0.16 | 1.38 ± 0.17 |
| HSP90 | −1.03 ± 0.07 | 1.03 ± 0.08 | −2.08 ± 0.11 | 2.53 ± 0.28 | 3.32 ± 0.14 |
| Importin β1 | 1.06 ± 0.11 | 1.03 ± 0.05 | 1.09 ± 0.15 | 1.06 ± 0.09 | 1.05 ± 0.07 |
| Kv3.1a | −1.79 ± 0.13 | −1.89 ± 0.12 | 2.14 ± 0.10 | −1.04 ± 0.14 | −1.03 ± 0.20 |
| Lipocortin 2 | 1.06 ± 0.07 | 1.04 ± 0.09 | 1.01 ± 0.07 | 1.04 ± 0.08 | 1.09 ± 0.09 |
| Neuritin | 1.07 ± 0.11 | 1.06 ± 0.16 | 1.00 ± 0.12 | 1.13 ± 0.13 | 1.08 ± 0.09 |
| NMP35 | −1.04 ± 0.06 | −1.04 ± 0.17 | 1.05 ± 0.14 | 1.10 ± 0.17 | 1.13 ± 0.11 |
| PEBP | 1.09 ± 0.08 | −1.03 ± 0.11 | −1.45 ± 0.11 | 1.14 ± 0.15 | 1.52 ± 0.16 |
| Peripherin | 2.85 ± 0.20 | 2.85 ± 0.13 | −1.32 ± 0.03 | 1.09 ± 0.19 | 1.11 ± 0.11 |
| Pgk1 | 1.03 ± 0.19 | 1.13 ± 0.16 | −1.13 ± 0.21 | 3.92 ± 0.13 | −1.10 ± 0.06 |
| Prdx1 | −1.03 ± 0.10 | 1.28 ± 0.04 | −1.10 ± 0.16 | 1.48 ± 0.17 | 1.36 ± 0.08 |
| Prdx6 | 1.08 ± 0.26 | 1.03 ± 0.21 | −1.23 ± 0.05 | 1.29 ± 0.11 | 1.14 ± 0.09 |
| RP-L11 | −1.13 ± 0.11 | −1.09 ± 0.12 | −1.04 ± 0.15 | 1.10 ± 0.13 | 1.15 ± 0.16 |
| RP-L22 | 3.10 ± 0.07 | 2.85 ± 0.08 | 1.26 ± 0.11 | −1.13 ± 0.21 | −1.98 ± 0.20 |
| RP-L24 | 2.66 ± 0.07 | 3.01 ± 0.07 | 1.16 ± 0.19 | −1.10 ± 0.11 | −1.05 ± 0.11 |
| RP-L37 | −1.08 ± 0.13 | −1.13 ± 0.17 | −1.09 ± 0.19 | −1.08 ± 0.19 | −1.13 ± 0.18 |
| RP-S17 | 2.50 ± 0.10 | 2.60 ± 0.10 | 1.09 ± 0.07 | −1.14 ± 0.09 | −1.12 ± 0.12 |
| RP-S23 | −1.13 ± 0.09 | −1.17 ± 0.08 | −1.15 ± 0.10 | 1.05 ± 0.10 | −1.01 ± 0.09 |
| RVDAC3 | −1.03 ± 0.08 | −1.04 ± 0.10 | −1.61 ± 0.17 | 1.38 ± 0.15 | −1.01 ± 0.08 |
| SOD1 | 1.13 ± 0.10 | 1.31 ± 0.16 | −1.01 ± 0.08 | 1.56 ± 0.12 | 1.58 ± 0.16 |
| Sp22 (DJ-1) | 1.12 ± 0.16 | 1.01 ± 0.14 | 2.28 ± 0.15 | 1.03 ± 0.16 | 2.19 ± 0.05 |
| Tα1 tubulin | 1.09 ± 0.08 | 1.15 ± 0.07 | −1.09 ± 0.06 | 1.44 ± 0.09 | 1.20 ± 0.11 |
| Thymosin β-4 | 1.95 ± 0.06 | 1.87 ± 0.07 | −1.64 ± 0.11 | 1.01 ± 0.11 | −1.82 ± 0.14 |
| UchL1 | 1.08 ± 0.12 | 1.03 ± 0.15 | 1.07 ± 0.12 | 1.09 ± 0.07 | 1.11 ± 0.19 |
| Vimentin | 3.56 ± 0.01 | 3.94 ± 0.21 | 1.01 ± 0.07 | 5.28 ± 0.16 | 4.72 ± 0.09 |
qPCR data for the axonal mRNAs tested for axonal level modulation by NGF, BDNF, NT3, MAG, and Sema3A are tabulated. Axonal levels of the mRNAs are compared with axons treated with controls (BSA for NGF, BDNF, and NT-3; IgG-Fc for MAG; and AP for Sema3A) and are displayed ± SD from three replicates. Values in bold indicate differences that were statistically significant based on P < 0.01 by the student Newman-Keul test. The other values are not statistically significant (P ≥ 0.01).