Figure 8.

Roles of Msk, Imp7, and Imp8 in nuclear import of Smads at basal state. (A) S2R+ cells transfected with Flag-Mad expression vector were subject to RNAi as indicated. After inducing the expression of Flag-Mad, the cells were analyzed by anti-Flag immunofluorescence staining (green). Bar, 10 μm. (B) HeLa cells were transfected with indicated siRNAs. 3 d later, cells were stained with anti-Smad2/3 antibody (red) without prior TGF-β treatment. Bar, 10 μm. (C) 2 d after HeLa cells were transfected with indicated siRNAs, the cells were further transfected with a Myc-Fox H1 expression vector. Double- immunofluorescence staining with anti-Myc (green) and anti-Smad2/3 (red) was performed 1 d after Fox H1 transfection with no TGF-β stimulation. Bar, 10 μm.