Figure 5.

Designed molecular beacons. (a) Based on the proposed elution mechanism for beacon 14a, two molecular beacons (cOT1 and cOT3) and three target oligonucleotides (OT1, OT3 and OT3b), were designed. (b) Proposed role of a hypothesized, immobilized secondary structure in the mechanism of elution. The hypothesized secondary structures of the selected and designed beacons are shown. (c) The elution characteristics of beacons cOT1 and cOT3 with targets OT1, OT3 and OT3b were assessed as in Figure 3a. The representations are as in Figure 3c.