Figure 8.

Wavelength-shifting beacons. (a) Sequence and predicted structure of a wavelength-shifting beacon based on beacon 14a. Representations are as in Figure 3c. The previously introduced fluorescein-dT is now labeled F1, while a second fluorophore (Texas Red) is F2. There are two DABCYL moieties, at positions Q1 (previously introduced) and Q2. (b) Fluorescence response at different wavelengths of beacon 14a to target OT2. The normalized signal-to-background ratio (percent change in fluorescence) is shown, as described in Materials and Methods. (c) Fluorescence response at different wavelengths of beacon 16c to target OT2.