Figure 5.

RPB7 affects the P bodies' number. Wild-type cells and their isogenic rpb7 ts derivatives, expressing DHH1-GFP (A and C) or PAT1-GFP (B) (see Table I), were allowed to proliferate at 30°C in a selective medium until late-log phase. Cultures were then shifted to 42°C for 45 min (A) or 60 min (B). Cells were washed 3× with water before they were visualized by fluorescent microscopy. (C) Average number of DHH1-GFP PBs per cell. Error bars indicate the SD from the mean values.