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. 2007 Sep 24;178(7):1145–1160. doi: 10.1083/jcb.200611086

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — hUPF3 localizes to P-bodies when NMD is inhibited. HeLa cells were transfected with a pcDNA3-hUpf3-FLAG or pcDNA3-hUpf3X-FLAG plasmid and either pGFP-Ge1, pYFP-hSmg6, or pYFP-hSmg7. 24 h after transfection, cells were incubated either with DMSO(−) or with 5 μM NMDI 1 for 20 h. In the merge, nuclei are visualized by Hoechst staining in blue. The white squares are magnifications of cell areas. Bars, 10 μm.