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. 2007 Sep 24;178(7):1145–1160. doi: 10.1083/jcb.200611086

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — Down-regulation of hUPF2 or hXRN1 does not lead to the accumulation of NMD factors in P-bodies. (A and C) The efficiency of the hUPF2 (A) or hXRN1 (C) down-regulation was evaluated by Western blot from 10 μg of total protein. The three leftmost lanes represent 2× dilutions of total extract from HeLa cells that were not transfected. (B) HeLa cells were transfected with pCFP-hDcp1a and either pCI-neo-FLAG-hUPF1, pcDNA3-hUPF3-FLAG, pcDNA3-hUPF3X-FLAG, or pmCMV-Gl Ter in the presence of siRNA luciferase (Luc) or siRNA hUPF2 (Upf2). (D) Same as B except siRNA hXRN1 replaced siRNA hUPF2, and pcDNA3-hUPF3X-FLAG was not tested. The white squares are magnifications of cell areas. Bars, 10 μm.