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. 2007 Sep 24;178(7):1145–1160. doi: 10.1083/jcb.200611086

Figure 8.

Figure 8.

NMDI 1 effect on the localization of NMD factors and substrates can be reproduced by using hUPF1 or hSMG5 mutants that prevent the phosphorylation of hUPF1. HeLa cells were transfected with pCFP-hDcp1a and pHA-hUpf1dNT (A), pHA-hSmg5dCT (B) or pHA-hSmg5DA (C), and either pCI-neo-FLAG-hUpf1, pcDNA3-hUpf3-FLAG, pcDNA3-hUpf3X-FLAG, pmCMV-Gl-Ter, pmCMV-GPx1-Ter, pYFP-hSmg5 (A), or pCI-neo-FLAG-hUpf1 (B and C). The white squares are magnifications of cell areas. Bars, 10 μm.