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. 2007 Sep 24;178(7):1193–1206. doi: 10.1083/jcb.200707080

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Myo2p is weakly processive and Myo4p ensembles move processively. Stepping traces and photobleaching of Myo2p-GFP and Myo4p-GFP as measure by FIONA. (A) Myo2p-GFP. (a–c) Stepping traces of representative Myo2p-GFP spots measure by FIONA and (d – f) respective photobleaching of the spots. Only 2% of spots show processive movement illustrated in (f). (B) Myo4p-GFP. (a–c) Stepping traces of representative Myo4p-GFP spots measure by FIONA and (d–f) respective photobleaching of the spots. Monomers (a, d) and potential dimers (b and e) of Myo4p-GFP do not move processively, but ensembles (c and f) of at least three Myo4p-GFPs move processively. Note that Myo2p-GFP and Myo4p-GFP were imaged with different cameras resulting in different spot intensities.