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. 2007 Sep 24;178(7):1237–1249. doi: 10.1083/jcb.200702184

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Expression pattern of LRP receptors in the chick embryonic spinal cord. In situ hybridization of ApoER2 (A), LRP2/megalin (B), and LRP4 receptors at E5 chick spinal cord (D), and LRP4 (C) VLDLR (E) at E4 chick spinal cord. (A) ApoER2 expression is confined to the midline, the lateral floor plate cells, and the ventral ventricular zone. Lower levels are detected at the medial floor plate. (B) Megalin is expressed at the ventricular zone, including the floor plate. (C) LRP4 is expressed at E4 in the dorsal third neural tube, at the ventricular zone, and laterally to the ventricular zone. (D) At E5, LRP4 expression spreads to the ventricular zone and the floor plate. (E) VLDLR is expressed in subpopulation of dorsal interneurons. Colabeling with Lhx2 reveals that VLDLR is expressed in dI1 neurons (not depicted). The dashed lines demarcate the spinal cord. Bars: (A, B, and D) 75 μm; (C and E) 100 μm.