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. 2007 Sep 24;178(7):1237–1249. doi: 10.1083/jcb.200702184

Figure 7.

Figure 7.

F-spondin is immobilized to the cell surface by the ApoER2 receptors. ApoER2 is required for the binding of myc-tagged TSR1-4 to the cell surface of COS cells. COS cells were transfected with TSR1-4–IRES–EGFP and nEGFP (A–C), TSR1-4–IRES–EGFP and ApoER2-IRES-nEGFP (D–F), a secreted form of ApoER2 (ApoER2ecto-IRES-nEGFP and TSR1-4–IRES–EGFP [G–I]), ApoER2ecto-IRES-nEGFP, ApoER2-IRES-EGFP, and TSR1-4–IRES–EGFP (J–L). At 48 h after the transfection, surface staining (without fixation) of the cells was preformed. TSR1-4 is bound to the cell surface, where it is coexpressed with ApoER2 (D–F). Bar, 5 μm.