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. 2007 Sep 24;178(7):1251–1264. doi: 10.1083/jcb.200703092

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Defects in endocytic compartment morphology and secretion of the vacuolar hydrolase CPY in a cofilin mutant. (A) Representative medial focal plane images of endocytic internalization of the lipophilic dye FM4-64 in wild-type and cof1-22 mutant cells. Vacuoles identified by brightfield microscopy are marked with red asterisks. (B) Colony blot assay for CPY secretion in wild-type cells and cof1-22, vps4Δ, and vps27Δ mutants grown at 25°C. Bar, 4 μm.