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. 2007 Sep 24;178(7):1279–1293. doi: 10.1083/jcb.200612053

Figure 2.

Figure 2.

RhoG and SGEF are expressed endogenously in endothelial cells and are localized to dorsal endothelial membrane protrusions. (A) Western blot analysis of tissue lysates of mouse brain (a positive control for SGEF), HUVECs, HeLa, and COS7 cells show the endogenous expression of SGEF (100 kD; top blot) and RhoG (18 kD; bottom blot). (B) Endothelial cells were transiently transfected with GFP–RhoG-Q61L (a) or GFP-SGEF (b) and stained for F-actin in red. Images represent the merge. Arrowheads show membrane ruffles.