. 2006 Dec 4;175(5):715–720. doi: 10.1083/jcb.200606113

Table I.

Localization of Vps4 substrates in wild-type, vps4Δ, and did2Δ mutant cells

Substrate	Complex	Wild type	vps4Δ	did2Δ
Vps23-GFP	ESCRT-I	Cytosolic^a	Punctate^b	Cytosolic^a
Vps36-GFP	ESCRT-II	Cytosolic^a	Punctate^b	Cytosolic^a
Snf7	ESCRT-III	Soluble^c	Membrane^d	Membrane^d
Vps24	ESCRT-III	Soluble^c	Membrane^d	Membrane^d
Bro1-GFP	NA	Cytosolic^a	Punctate^b	Punctate^b
Doa4-GFP	NA	Cytosolic^a	Punctate^b	Punctate^b
Vta1-GFP	With Vps60	Cytosolic^a	Punctate^b	Cytosolic^a

NA, not applicable.

Predominantly cytosolic GFP signal.

Punctate GFP signal adjacent to the vacuole with a reduction in cytosolic signal.

Soluble as determined by subcellular fractionation.

Membrane bound as determined by subcellular fractionation.