Table I.

Genotypes of all strains used and generated in this study

Name	Genotype	Source
ELY126 (FLY95)	MATα ura3-52 leu2-3,112 trp1Δ1 his3Δ200 (S288C)	F. Luca (University of Pennsylvania, Philadelphia, PA)
ELY402 (FLY93)	MAT a ura3-52 leu2-3,112 trp1Δ1 his3Δ200 (S288C)	F. Luca
ELY140 (DDY2362)	MAT a CBK1-3xHA∷HIS3	D. Drubin (University of California, Berkeley, Berkeley, CA)
ELY390	MATα cbk1S570A-3xHA∷HIS3
ELY461	MAT a cbk1S570A-3xHA∷HIS3
ELY437	MATα cbk1T743A-3xHA∷HIS3
ELY462	MAT a cbk1T743A-3xHA∷HIS3
ELY426	MATα cbk1D475A-3xHA∷HIS3
ELY463	MAT a cbk1D475A-3xHA∷HIS3
ELY132	MATα cbk1Δ∷KanMX
ELY447	MATα MOB2-13xMyc∷TRP1
ELY417	MAT a CBK1-3xHA∷HIS3 MOB2-13xMyc∷TRP1
ELY418	MATα cbk1S570A-3xHA∷HIS3 MOB2-13xMyc∷TRP1
ELY448	MATα cbk1T743A-3xHA∷HIS3 MOB2-13xMyc∷TRP1
ELY449	MATα cbk1D475A-3xHA∷HIS3 MOB2-13xMyc∷TRP1
ELY398	MAT a CBK1-3xHA∷HIS3 kic1Δ∷TRP1
ELY395	MAT a CBK1-3xHA∷HIS3 hym1Δ∷TRP1
ELY396	MAT a CBK1-3xHA∷HIS3 tao3Δ∷TRP1
ELY397	MAT a CBK1-3xHA∷HIS3 ace2Δ∷TRP1
ELY399	MAT a CBK1-3xHA∷HIS3 mob2Δ∷TRP1
ELY436	MAT a CBK1-3xHA∷HIS3 sog2Δ∷TRP1
ELY410	MATα ACE2-GFP∷KanMX
ELY464	MAT a cbk1S570A-3xHA∷HIS3 ACE2-GFP∷KanMX
ELY465	MAT a cbk1T743A-3xHA∷HIS3 ACE2-GFP∷KanMX
ELY451	MATα cbk1D475A-3xHA∷HIS3 ACE2-GFP∷KanMX
ELY466 (FLY869)	MAT a CBK1-GFP∷KanMX	F. Luca
ELY468	MATα cbk1S570A-GFP∷TRP1
ELY467	MATα cbk1T743A-GFP∷KanMX
ELY469	MATα cbk1D475A-GFP∷KanMX
ELY535	MAT a CBK1-GFP∷KanMX SEC63-tomato∷HIS3
ELY536	MATα cbk1S570A-GFP∷TRP1 SEC63-tomato∷HIS3
ELY537	MAT a /MATα CBK1-GFP∷KanMX cbk1D475A-HA∷HIS3

All strains are derived from ELY126 and ELY402.