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. 2006 Dec 4;175(5):791–802. doi: 10.1083/jcb.200606017

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Wnt11-induced accumulations of Fz7 modulate cell contact persistence. (A–C) Time-series of separating cells in the animal pole assay expressing either a combination of fz7-yfp (60 pg mRNA) and wnt11 (10 pg mRNA; A; Video 1), fz7-yfp alone (60 pg mRNA; B; Video 3), or lyn-yfp (25 pg mRNA; C; Video 4). Cells were “back-tracked” from the time point of separation (0′). Z sections shown from 3D, two-photon imaging. Time intervals are 130 (A and B) and 123 s (C); contact sites are marked by arrowheads. (D) Ratio of length of Fz7 accumulation/total cell contact length at the first video time point and last time point before separation (n = 26 out of 4 videos). Error bars represent the SEM. (E) Diagram of contacting cells containing Lyn-YFP or Fz7-YFP (control, black) versus Wnt11 + Fz7-YFP (white) at the last time point before separation. (F) Average contact angle between contacting cells at the last time point before separation. Number of cells analyzed was as follows: 34 (Fz7), 31 (Lyn), and 27 (Fz7 + Wnt11) out of 4 videos per condition. Asterisks mark statistically significant differences (P < 0.05). (G) Relative distribution of contact times from randomly chosen pairs of separating cells measured from start of the time-lapse until cell separation. Number of cells analyzed was as follows: 38 (Fz7), 32 (Lyn), and 25 (Fz7 + Wnt11) out of 4 videos per condition. (G′) Percentage of cells not separating within a 75-min time frame. Number of cells analyzed was as follows: 42 (Fz7), 43 (Lyn), and 36 (Fz7 + Wnt11) out of 4 videos each. (H) Ratio of cells contacting for >30 min (including nonseparating cells)/cells separating for <30 min from start of video. Calculated from data shown in G and G′. (I) Dynamic reduction of cell contact length during separation (Lyn, black; Fz7, gray; Fz7 + Wnt11, white) and reduction of Fz7 accumulation length (Fz7 + Wnt11, red). Graphs represent an average of all separation events measured per condition. Number of cells analyzed was as follows: 19 (Fz7), 26 (Lyn), and 23 (Fz7 + Wnt11) out of 4 videos per condition. t = 0 min is the first time point of separation. Bars, 10 μm. Videos 1, 3, and 4 are available at http://www.jcb.org/cgi/content/full/jcb.200606017/DC1.