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. 2006 Dec 4;175(5):791–802. doi: 10.1083/jcb.200606017

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Fmi2 colocalizes with Fz7 and Wnt11 at cell contacts and increases persistence. (A–F) Animal pole assay in fixed embryos expressing a mix of fz7-cfp (110 pg mRNA) and fmi2-yfp (50 pg DNA; A–C), or a mix of wnt11-cfp (90 pg mRNA), fz7 (50 pg mRNA), and fmi2-yfp (50 pg DNA; D–F). (A–C) Fz7-CFP (green) and Fmi2-YFP (red) colocalize in accumulations (arrowheads) at cell contacts. (D–F) Wnt11-CFP (green) and Fmi2-YFP (red) in the presence of exogenous Fz7 colocalize in accumulations (arrowheads) at cell contacts. (G) Time series of separating cells expressing Fmi2-YFP in the animal pole assay (Video 5). Fmi2-YFP accumulates (arrowheads) and remains at contacts until cell separation. Time series was obtained as described in Fig. 5, at time intervals of 171 s. (H) Relative distribution of cell contact times for separating cells expressing either fmi2-yfp (black), wnt11 + fz7-yfp (white), a combination of wnt11 + fz7-yfp, and MOs targeted against fmi1a, fmi1b, and fmi2 (4 ng/MO; gray; Video 6). Cell contact times were measured as described in Fig. 5. Number of cells analyzed was as follows: 24 (Fmi2), 25 (Fz7 + Wnt11), and 29 (Fz7 + Wnt11 + FmiMO) out of 4 videos per condition. (H′) Percentage of cells not separating within 75 min. Number of cells analyzed was as follows: 40 (Fmi2), 36 (Fz7 + Wnt11), and 35 (Fz7 + Wnt11 + FmiMO) out of 4 videos per condition. (H") Ratio of cells contacting for >30 min (including nonseparating cells)/cells separating for <30 min from start of video. Calculated from data shown in H and H′. (I and I′) Dynamic reduction of cell contact length during separation (I′), measured as described in Fig. 5. Number of cells analyzed was as follows: 19 (Fz7), 26 (Lyn), 23 (Fz7 + Wnt11), 27 (Fz7 + Wnt11 + FmiMO), and 25 (Fmi2) out of 4 videos per condition. Velocity of cell contact shrinkage (I) representing the slope of the last time point before separation to t = 0 in I′. Asterisks mark significant difference with P < 0.05. Error bars represent the SEM. Bars, 10 μm. Videos 5 and 6 are available at http://www.jcb.org/cgi/content/full/jcb.200606017/DC1.