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. 2006 Dec 18;175(6):869–880. doi: 10.1083/jcb.200604005

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — Ectopic expression of histone H1 does not prevent RasG12V-induced senescence. WI-38 cells were first infected with retrovirus (retro) or lentivirus (lenti) expressing histone H1 under the control of the indicated promoters and were infected with RasG12V-expressing retrovirus. Cell lysates were prepared from transfected cells at day 7 after drug selection and analyzed by immunoblotting. Transfected cells were scored for the percentages of BrdU- and SAHF-positive cells at day 3 after drug selection (bottom; n = 200). CMV, cytomegalovirus; LTR, long terminal repeat.