Figure 5.

Unspliced introns impact on protein products. Unspliced introns have variable effects on translation products. (a) The lack of splicing of the second intron yields a protein of similar length, albeit a different C-terminus. (b) Two different overlapping introns, varying at the donor splice junction, encode an integer number of codons and splicing removes internal segments from the protein. (c) Intron splicing alters the reading frame, providing a different and shorter C-terminus. (d) The lack of splicing truncates the protein sequence due to a stop codon encountered within the unspliced intron sequence.