Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Dec 18;175(6):901–911. doi: 10.1083/jcb.200608073

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Effect of the IP₃R ligand-binding domain on mitochondrial Ca²⁺ uptake. (A and C) HeLa cells were transfected with mitochondrially targeted (mtAEQmut; top) and cytosolic aequorin (bottom). Control traces are shown in black; traces from cells cotransfected with the IP₃R-LBD_224-605 (A) and the IP₃R-LBD_224-605 K508 mutant (C) are shown in gray. Traces are representative of >15 experiments from >5 preparations. (B) Effect of the cytosolic-, OMM-, and ER-targeted IP₃R-LBD_224-605 on peak mitochondrial and cytosolic Ca²⁺ responses (top and bottom, respectively). (D) Effect of the OMM-IP₃R-LBD_224-605 (K508A), the IP₃-binding PH domain of the p130 PLC-like protein (OMM-p130-PH), and the OMM targeted N-terminal (1-604 aa) part of the IP₃R (OMM-IP₃R-LBD_1-604), on mitochondrial (top) and cytoplasmic Ca²⁺ responses (bottom) after 100 μM histamine stimulation. Data in B and D were normalized to mean of the control group. Mean ± SEM of variation is shown as percentage. Cells were transfected, and [Ca²⁺] was measured as described in Materials and methods. Values are shown. *, P < 0.05; **, P < 0.01. For absolute values see Table S1, available at http://www.jcb.org/cgi/content/full/jcb.200608073/DC1.