Skip to main content
. 2006 Dec 18;175(6):937–946. doi: 10.1083/jcb.200604073

Figure 2.

Figure 2.

STAT5A and MgcRacGAP simultaneously entered the nucleus. (A) Stoichiometry of the association between MgcRacGAP and p-STAT5A or total STAT5A in the cytoplasm and nucleus. IL-3–starved Ba/F3 cells were stimulated with IL-3 for the times indicated, and cytosol and nuclear extracts were prepared as described previously (Nakamura et al., 2002). 10 μg of protein for each of the extracts was immunodepleted with the anti-MgcRacGAP, anti-STAT5A, or control antibody, followed by immunoblotting with the anti–p-STAT5 and anti-STAT5A antibodies (a–d). The immunoprecipitates were also examined by Western blotting with the anti–p-STAT5 and anti-STAT5 antibodies (e–h). (B) STAT5A and MgcRacGAP translocated into the nucleus upon ITD-Flt3 stimulation in 293T cells. Cells were transfected with pME/STAT5A-Flag together with pMKIT (MOCK; top) or pMKIT/ITD-Flt3 (bottom). After 36 h, cells were immunostained with the anti-MgcRacGAP and anti-Flag antibodies and viewed using a Fluoview FV300 confocal microscope. Bar, 10 μm.