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. 2006 Dec 18;175(6):937–946. doi: 10.1083/jcb.200604073

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — The nuclear translocation of p-STAT5 was not observed in the Rac1-knockout mouse embryonic fibroblasts. (A) The nuclear translocation of ITD-Flt3–induced p-STAT5 was impaired in the Rac2^−/−Rac1^flox/flox fibroblasts. The Rac2^−/−Rac1^flox/flox fibroblasts were transduced with a control pMX-IG (a and b) or pMX-IG-Cre (c and d) retrovirus vector. After 3 d, cells were transiently cotransfected with pME/STAT5A-Flag and MOCK (a and c) or pMKIT/ITD-Flt3 (b and d). After 36 h, the cells were fixed and immunostained with the anti-Flag antibody (a–d, left) or anti–p-STAT5 antibody (e and f, left). Bars, 10 μm. (B) Expression of Rac1 was depleted in the Rac2^−/−Rac1^flox/flox fibroblasts by Cre recombinase. Expression of α-tubulin and Rac1 was examined in the Rac2^−/−Rac1^flox/flox fibroblasts retovirally transduced with a control pMX-IG or pMX-IG-Cre.