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. 2006 Dec 18;175(6):993–1003. doi: 10.1083/jcb.200605114

Figure 4.

Figure 4.

β4 sustains HGF-induced anchorage-independent growth through the activation of Shp2, Src, and ERK. (A) Western blot analysis showing expression of the indicated cDNAs in MDA-MB-435 cells subjected to soft agar assays. Filters probed with anti-Shp2 and -Src antibodies were deliberately underexposed to better visualize the overexpression of exogenous proteins. (B) Representative images and quantitation of soft agar assays in MDA- MB-435 cells with ectopic expression of the indicated proteins in the absence (NS) or presence of 30 ng/ml HGF. Pictures show colony aspect at high magnification, whereas the graph reports colony numbers as counted at low magnification over the entire microscopic field. Light bars, no stimulation; dark bars, HGF. (C) Representative images and quantitation of soft agar assays in MDA-MB-435 cells expressing β4 that were either left untreated (NS) or were treated with HGF in the absence or presence of the Src inhibitor PP2 or the ERK inhibitor PD98059. Data are the means ± SEM (error bars) of two independent experiments performed in triplicate. **, P < 0.01 with respect to all other transfectants. Bars, 400 μm.