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. 2006 Dec 18;175(6):1005–1015. doi: 10.1083/jcb.200606062

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — Skin-derived spheres contain cells that express the NCSC markers p75 and Sox10. Spheres were generated from murine adult trunk skin (A) and human adult thigh skin (B). Spheres passaged >22 times were allowed to spread on FN-coated plates (C–F) and fixed after 8 h. Immunocytochemical analysis revealed that both murine and human skin–derived spheres contain numerous cells expressing p75 (visualized by Alexa 488 fluorescence, green) and Sox10 (revealed by Cy3 fluorescence, red; E and F). The arrow shows a double-positive cell, and the arrowhead shows a negative cell. (C and D) Corresponding DAPI staining.