Figure 7.
GLIS3 is able to function as a transcriptional repressor and activator. (A) (UAS)5-LUC, pCMVβ and pM-GLIS3 plasmid DNAs were co-transfected into CV-1, CHO and 293 cells. After 30 h, cells were assayed for luciferase (LUC) and β-galactosidase activity as described in Materials and Methods. The relative LUC reporter activity was calculated and plotted. (B) Repression of basal transcription by Gal4(DBD)–GLIS3 and its reversal by GLIS3. CV-1 cells were co-transfected with (UAS)5-LUC, pCMVβ and pM or different amounts of pM-GLIS3 in the presence or absence of pCMV-mycGLIS3 expression plasmid as indicated. Reporter activity was measured 30 h later.