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Effect of various N- and C-terminal deletions on the transcriptional activity of GLIS3. CHO cells were co-transfected with (UAS)5-LUC, pCMVβ and pM or pM-GLIS3 containing various C- (A) or N-terminal (B) deletions in GLIS3 as indicated. Forty-eight hours after transfection, cells were assayed for LUC and β-galactosidase activity as described in Materials and Methods. The relative LUC activity was calculated and plotted.
