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. 2007 Oct 8;179(1):75–86. doi: 10.1083/jcb.200704166

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — Direct interaction of CSSR with unfolded proteins as monitored by inhibition of in vitro aggregation. (A) Bacterially expressed MBP-CSSR, its mutant version, or unfused MBP were purified; run on 10% SDS-PAGE gel (1 μg of protein per lane); and stained with Coomassie blue. (B and C) At time 0, 25 μM of luciferase (B) or 50 μM of citrate synthase (C) in guanidine HCl–denaturing solution was 50- (for luciferase) or 66-fold (for citrate synthase) diluted into assay buffer containing MBP-CSSR, its mutant version, unfused MBP (2 μM each for luciferase or 0.5 μM each for citrate synthase), or buffer only. Turbidity of the sample mixtures was monitored, normalized against the maximal value of the buffer sample, and presented as aggregation.