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. 2007 Oct 8;179(1):87–99. doi: 10.1083/jcb.200702082

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Effect of selective Δψ and ΔpH gradient collapse on pre-SufI and spTorA-GFP transport efficiency. (A) Correlation between Δψ_avg and pre-SufI transport efficiency. This anti-SufI immunoblot shows standard transport assays (Fig. 1 C) with different concentrations of nigericin (Nig.) alone (lanes 6–8), or with both nigericin and valinomycin (Val.) (lanes 11–14). The control reaction (lane 4) had no NADH added. Transport efficiency was calculated based on the transport observed in the absence of ionophores (lanes 5 and 9). Same key as Fig. 5 C. (B) Effect of selective gradient collapse on pre-SufI transport efficiency. This anti-SufI immunoblot shows standard transport assays with 10 μM nigericin (N), 10 μM valinomycin (V), and/or 25 mM NaSCN (S) added before reaction initiation (0) or 30 s after reaction initiation (30 s), as indicated. The control reactions (C) had no uncouplers. Values are given in the bar graph as mean ± SD (n = 3). Transport efficiency was calculated based on the transport observed in the absence of uncouplers (lane 6). (C) Effect of selective gradient collapse on spTorA-GFP transport efficiency. The same experiment as panel B using spTorA-GFP instead of pre-SufI.