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. 2007 Oct 8;179(1):87–99. doi: 10.1083/jcb.200702082

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Copyright © 2007, The Rockefeller University Press

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Figure 9. — Effect of delayed addition of spTorA-GFP on pre-SufI transport efficiency. This anti-SufI immunoblot shows the amount of pre-SufI transported when spTorA-GFP (500 nM) was added at various times after reaction initiation (n = 3). The control lanes show the amount of pre-SufI transported after 30 min in the presence (+) and absence (−) of NADH with no spTorA-GFP. Transport efficiency was calculated based on the transport observed in the +NADH control lane. All assays were conducted under standard transport conditions (Fig. 1 C).