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. 2003 Oct 1;31(19):5627–5634. doi: 10.1093/nar/gkg781

Figure 4.

Figure 4

Modulation of AP-1 binding activity in U937 cells depending on TPA treatment. Left, extracts from untreated or TPA-induced U937cells (as indicated) were assayed for AP-1 DNA-binding activity using the TRE site of the collagenase promoter (AP-1/Coll, lanes 1–4). Right, same cell extracts were assessed for an alternate binding capacity of Ku heterodimer on a 18mer linear dsDNA probe (lanes 5 and 6). Added competitors are mentioned in the figure as non-specific oligonucleotides (N.S.), AP-1/Coll. oligonucleotides (AP-1/Coll.) or covalently closed circular DNA (cccDNA). Conditions for band shift reaction were identical to those used in Figure 3. TPA-induced AP-1 complex and Ku–DNA complex are respectively identified by single and double asterisks.