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. 2003 Oct 1;31(19):5627–5634. doi: 10.1093/nar/gkg781

Figure 5.

Figure 5

Deficit of TPA-induction of the hMSH2 expression associated to the loss of c-Jun transactivation activity. U937 cells carrying a dominant negative form of c-Jun (dnJun) were cultured in the presence (+) or absence (–) of 50 nM of TPA. (A) Cell extracts were analysed for hMSH2 and, as a control, PCNA protein expression by western blot analysis. (B) RT–PCR experiments were completed on total RNAs extrated from untreated or treated U937 cells, carrying the dn-Jun plasmid (dnJun) or the vector alone (U937), with procedures similar to those described in Figure 2. Left, co-amplification of HB2 (HB2) and procaryotic control (control) mRNAs. Right, co-amplification of hMSH2 (hMSH2) and procaryotic control mRNAs.