Figure 1.
Reconstitution of telomerase activity in G3 Terc+/−* mice compared with their G3 Terc−/− siblings. Telomerase is successfully reconstituted in two independent MEF cultures from G3 Terc +/−* mice compared with their telomerase-deficient G3 Terc −/− littermates. Two wild-type MEFs are also included as a positive control for telomerase activity. The protein concentration used is indicated on top of each lane. Extracts were treated (+) or not treated (−) with RNase as a negative control. An internal control (IC) for PCR efficiency was also included.