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. 2007 Oct 22;179(2):277–290. doi: 10.1083/jcb.200704141

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — Pronounced rescue of short telomeres in Terc-reconstituted and Terc-deficient keratinocytes and splenocytes. (A) Telomere length histograms obtained by Q-FISH on tail skin sections. The histogram shown is representative of three independent age-matched (10 mo old) pairs of Terc ^+/− and telomerase-reconstituted G3 Terc ^+/−* mice (see B). Red lines facilitate visualization of short (<60 a.u.f.) and long (>600 a.u.f.) telomeres. The total number of telomere dots used for the quantification and the percentage of short and long telomeres are indicated. Note very similar telomere length distributions in age-matched Terc ^+/− and telomerase-reconstituted G3 Terc ^+/−* mice. (B) Mean telomere length is indistinguishable between age-matched Terc ^+/− and telomerase-reconstituted G3 Terc ^+/−* mice (P = 0.98). Concomitantly, these mice show similar percentages of short telomeres (P = 0.22) and long telomeres (P = 0.70). Data are mean values ± SEM for three independent pairs of G3 Terc ^−/− and G3 Terc ^+/−* mice. (C) Telomere length histograms obtained by Q-FISH directly on metaphases of freshly isolated splenocytes. The histogram shown is representative of three age-matched (1–2 yr old) pairs of Terc ^+/− and telomerase-reconstituted G4 Terc ^+/−* mice. In this case, arbitrary units of fluorescence were converted into kilobases as described in Materials and methods. Red lines facilitate visualization of short (<10 Kb) and long (>50 Kb) telomeres. The total number of individual telomeres used for the quantification and the percentage of short and long telomeres are indicated. Note very similar telomere length distributions in age-matched Terc ^+/− and telomerase-reconstituted G4 Terc ^+/−* mice. (D) Mean telomere length is indistinguishable between age-matched Terc ^+/− and telomerase-reconstituted G4 Terc ^+/−* mice (P = 0.31). Concomitantly, these mice show similar percentages of signal-free ends (P = 0.22), short telomeres (<10 Kb; P = 0.59), and long telomeres (>50 Kb; P = 0.75). Data are mean values ± SEM for three independent Terc ^+/− and three independent G4 Terc ^+/−* mice.